EcoRI 内切酶
EcoRI
CAS: 80498-17-5
Molecular Formula:
EcoRI 内切酶 - Names and Identifiers
EcoRI 内切酶 - Physico-chemical Properties
| Appearance | buffered aqueous glycerol solution |
| Storage Condition | −20°C |
EcoRI 内切酶 - Risk and Safety
| WGK Germany | 3 |
| RTECS | RF0757500 |
EcoRI 内切酶 - Reference
| Reference Show more | Note: part of our products can only provide part of the information, our company does not guarantee the authority of the information provided, only for the customer reference Exchange research. storage conditions: -20°C heat inactivation: loss of activity by heating at 65°C for 20 minutes methylation effect: dam methylation insensitive, dcm methylation insensitive, cpG methylation sensitive ligation and re-cleavage: After more than 10 times of enzymatic digestion, 95% of DNA fragments can be ligated and re-bound storage conditions: 10 mM potassium phosphate (pH 7.4,25°C),300 mM NaCl,1 mM EDTA,1 mM DTT,0.2 mg/mL BSA,0.15% Triton X-100,50% glycerol enzyme activity definition: 1 U enzyme is at 1 h,37°C, enzyme required for hydrolysis of 1 μg λ DNA in 50 μL total reaction volume incubation: 37°C general buffer: 10X Single buffer optimal buffer: 10X EcoR I buffer(500 mM Tris-HCl(pH 7.5),100 mM MgCl2,1000 mM NaCl,0.2% Triton X-100,1 mg/ml BSA) Source: recombinant E. Coli carrying ecoRIR cloned gene from E. Coli recognition site: 5 '-G AATTC-3' Technical specification |
EcoRI 内切酶 - Instructions for Use
The use of high enzyme concentrations and non-optimal buffers may lead to star activity. Do not incubate for a long time when using BSA. To obtain 100% activity, BSA should be added to the 1X reaction mixture to a final concentration of 100 μg/ ml.
Last Update：2023-08-16 21:32:38
EcoRI 内切酶 - Application
Enzymatic digestion
Last Update：2023-08-16 21:32:38
EcoRI 内切酶 - Characteristics
All endonucleases had 100% activity in both the optimum buffer and the uniform buffer; The buffer was 100% compatible with downstream experimental applications.
Last Update：2023-08-16 21:32:38
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Product Name: EcoRI Visit Supplier Webpage Request for quotation
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
View History
EcoRI 内切酶
VAT BROWN G (VAT BROWN 68)
4-BROMPHENYLISOCYANIDE
4-Methylaminobenzoic acid
2-cyanomorpholine HCl
(5S)-5-hydroxy-1-(4-hydroxy-3-methoxy-phenyl)tetradecan-3-one
BTD-TRPC5 activator
CS-506
1,3-DIAMINOPROPANE FOR SYNTHESIS
Short-leaved hematoxylic acid
VAT BROWN G (VAT BROWN 68)
4-BROMPHENYLISOCYANIDE
4-Methylaminobenzoic acid
2-cyanomorpholine HCl
(5S)-5-hydroxy-1-(4-hydroxy-3-methoxy-phenyl)tetradecan-3-one
BTD-TRPC5 activator
CS-506
1,3-DIAMINOPROPANE FOR SYNTHESIS
Short-leaved hematoxylic acid